Board Thread:Rebirth: Applications/@comment-8914761-20141004174402/@comment-28861328-20141007012403

Yes. Susanoo awakens when you have mastered the "double Mangekyou", which basically translates to mastering both eyes. Kakashi gained it by having Kamui in both eyes, Shisui flat out said in an anime episode (his Susanoo is anime-only anyways), that he had Kotoamatsukami in both eyes, Sasuke has Amaterasu and Kagutsuchi in his eyes, and Itachi has Tsukuyomi and Amaterasu. We aren't sure what Madara used.